- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-23355h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Oocyte-expressed protein homolog,OOEP
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Oocyte- expressed protein homolog, OOEP ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
OOEP,C6orf156; FLOPED; HOEP19; KHDC2; KH homology domain containing 2; KH homology domain-containing protein 2; oocyte and embryo protein 19; oocyte expressed protein homolog; oocyte- and embryo-specific protein 19; oocyte-expressed protein homolog; oocyte expressed protein
Search name
Human OOEP ELISA KIT ,Human C6orf156 ELISA KIT ,Human FLOPED ELISA KIT ,Human HOEP19 ELISA KIT ,Human KHDC2 ELISA KIT ,Human KH homology domain containing 2 ELISA KIT ,Human KH homology domain-containing protein 2 ELISA KIT ,Human oocyte and embryo protein 19 ELISA KIT ,Human oocyte expressed protein homolog ELISA KIT ,Human oocyte- and embryo-specific protein 19 ELISA KIT ,Human oocyte-expressed protein homolog ELISA KIT ,Human oocyte expressed protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Oocyte- expressed protein homolog, OOEP beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Oocyte- expressed protein homolog, OOEP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Oocyte- expressed protein homolog, OOEP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Oocyte- expressed protein homolog, OOEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Oocyte- expressed protein homolog, OOEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
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文献和实验Purification of Baculovirus-Expressed Human DNA Topoisomerase I
of truncated forms of the protein (Madden and Champoux, unpublished observations). In addition, the E. coli -expressed htopo I is very unstable, with proteolytic breakdown products nearly as abundant as those of the full-length protein (6 ). This is true
Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
, 5 ng in vitro-transcribed AChE mRNA directed the production of catalytically active recombinant human acetylcholinesterase (rHAChE), at levels of approx 10-fold above background oocyte levels (Table 6 ). Oocyte-produced rHAChE was sensitive to the AChE
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Protein kinase A (PKA) activity is regulated by intracellular cyclic adenosine mono-phosphate. Conventional protein kinase assays after cell lysis are hence not suitable for analyzing PKA activities. In this chapter, we describe a new method
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