Human Homeobox protein OTX2,OT

Human Homeobox protein OTX2, OTX2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Homeobox protein OTX2,Orthodenticle homolog 2, OTX2,homeobox protein OTX2; CPHD6; MCOPS5; orthodenticle homolog 2; orthodenticle homeobox 2

Search name

Human Homeobox protein OTX2 ELISA KIT ,Human Orthodenticle homolog 2 ELISA KIT ,Human OTX2 ELISA KIT ,Human homeobox protein OTX2 ELISA KIT ,Human CPHD6 ELISA KIT ,Human MCOPS5 ELISA KIT ,Human orthodenticle homolog 2 ELISA KIT ,Human orthodenticle homeobox 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human homeobox protein otx2,Homeobox protein OTX2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homeobox protein OTX2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Homeobox protein OTX2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Homeobox protein OTX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Homeobox protein OTX2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.