- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-23283h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
P2X purinoceptor 6,P2RX6
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human P2X purinoceptor 6, P2RX6 ELISA KIT
Product Name:Human P2X purinoceptor 6, P2RX6 ELISA KIT
Packing:96T
Catalog No.:ELI-23283h
Gene Name:P2RX6
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:P2X purinoceptor 6(P2RX6
Alternative Name:P2RX6,P2X purinoceptor 6(P2RX6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human P2X purinoceptor 6, P2RX6 ELISA KIT allows for the in vitro quantitative determination of P2X purinoceptor 6(P2RX6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human P2X purinoceptor 6, P2RX6 ELISA KIT has been pre-coated with an P2X purinoceptor 6(P2RX6 antibody specific to P2X purinoceptor 6(P2RX6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for P2X purinoceptor 6(P2RX6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain P2X purinoceptor 6(P2RX6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of P2X purinoceptor 6(P2RX6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验can be amplified. Aim PCR amplification of the P2X2-specific fragment will be performed using the following primers which introduces restriction sites in order to facilitate manipulation of the amplified DNA product at a later stage
7.3 Cell子刊从不同的角度看CRISPR-Cas9：让“剪刀”卡住
靶点的药物研发提供了新的思路。这一研究成果公布在6月24日的Nature Communications杂志上，文章通讯作者为华中科技大学生命学院刘剑峰教授和法国国家科学研究中心功能基因研究所Rondard P教授。原文检索：Rearrangement of the transmembrane domain interfaces associated with the activation of a GPCR hetero-oligomer⑥ eLife：生理环境中的二价阳离子对P2X3受体的调节机制P2X
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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