- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-22341h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Histone H2A deubiquitinase MYSM1,MYSM1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT
Product Name:Human Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT
Packing:96T
Catalog No.:ELI-22341h
Gene Name:MYSM1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Histone H2A deubiquitinase MYSM1(MYSM1
Alternative Name:MYSM1,Histone H2A deubiquitinase MYSM1(MYSM1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT allows for the in vitro quantitative determination of Histone H2A deubiquitinase MYSM1(MYSM1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT has been pre-coated with an Histone H2A deubiquitinase MYSM1(MYSM1 antibody specific to Histone H2A deubiquitinase MYSM1(MYSM1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone H2A deubiquitinase MYSM1(MYSM1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone H2A deubiquitinase MYSM1(MYSM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone H2A deubiquitinase MYSM1(MYSM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验工要准确，但是由于经费问题，现在国内使用此法人工计数的不在少数，因此这样可信度就不高。 如果要探讨调亡机理，可以结合免疫组化法来检测如bcl-2,fas等调亡蛋白的表达。 ELISA方法检测淍亡的原理 DONGHAIJU 细胞凋亡时，Ca2+,Mg2+离子依赖的内源性核酸内切酶将双链DNA从各核小体间连接区裂断，产生单或寡合苷酸小体，各核小体的DNA与核组蛋白H2A,H2B,H3,H4形成紧密的复合物而对内源性核酸酶有抵抗使之不被内源性核酸酶裂解。主要使用单克隆抗DNA抗体和抗组蛋白抗体直接检
Use of In Vivo Biotinylation for Chromatin Immunoprecipitation
ratio). (A ) Silver stain of biotinylated protein expressed in HEK 293 cells, separated by SDS‐PAGE and washed with either standard buffer or 2% SDS. (B ) Interaction of histone H2A.Z with the total genome of NIH3T3 cells. NIH3T3 cells were transiently
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