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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Proteasome activator complex subunit 1,PSME1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Proteasome activator complex subunit 1, PSME1 ELISA KIT
Product Name:Human Proteasome activator complex subunit 1, PSME1 ELISA KIT
Packing:96T
Catalog No.:ELI-22271h
Gene Name:PSME1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Proteasome activator complex subunit 1(PSME1
Alternative Name:PSME1,Proteasome activator complex subunit 1(PSME1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Proteasome activator complex subunit 1, PSME1 ELISA KIT allows for the in vitro quantitative determination of Proteasome activator complex subunit 1(PSME1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Proteasome activator complex subunit 1, PSME1 ELISA KIT has been pre-coated with an Proteasome activator complex subunit 1(PSME1 antibody specific to Proteasome activator complex subunit 1(PSME1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Proteasome activator complex subunit 1(PSME1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proteasome activator complex subunit 1(PSME1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Proteasome activator complex subunit 1(PSME1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assays for Proteasome Assembly and Maturation
The 20S proteasome is a complex multisubunit protease that is present in all phylae of life. Eukaryotic 26S proteasomes, which are composed of 20S proteasomes and 19S activator complexes, mediate the degradation of ubiquitylated proteins
to establish the rules governing structure/activity relationships of the human 20S proteasome. This chapter describes the use of a combination of proteomic approaches to characterize the human 20S proteasome subunit isoforms separated by 2D gel electrophoresis
Studies of the Ubiquitin Proteasome System
of Small Interfering RNAs (siRNAs) to Reduce Expression of Ubiquitin‐Proteasome System Proteins in Cells Basic Protocol 13: Localizing Degradation to the Proteasome The NEDD8 Conjugation System Basic Protocol 14: Purification of APP‐BP1/Uba3 Complex (E1
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