相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
NF-kappa-B-repressing factor,NKRF
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human NF- kappa- B- repressing factor, NKRF ELISA KIT
Product Name:Human NF- kappa- B- repressing factor, NKRF ELISA KIT
Packing:96T
Catalog No.:ELI-22122h
Gene Name:NKRF
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:NF-kappa-B-repressing factor(NKRF
Alternative Name:NKRF,NF-kappa-B-repressing factor(NKRF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NF- kappa- B- repressing factor, NKRF ELISA KIT allows for the in vitro quantitative determination of NF-kappa-B-repressing factor(NKRF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NF- kappa- B- repressing factor, NKRF ELISA KIT has been pre-coated with an NF-kappa-B-repressing factor(NKRF antibody specific to NF-kappa-B-repressing factor(NKRF .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NF-kappa-B-repressing factor(NKRF and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-kappa-B-repressing factor(NKRF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of NF-kappa-B-repressing factor(NKRF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验The nuclear factor-kappa B (NFκB) family of transcription factors has emerged as a signaling pathway that figures prominently in a cell’s initial response to a plethora of inflammatory stimuli. Modified lipids, oxidative stress, bacterial
Activation of Nuclear Factor-B
Nuclear factor-κB (NF-κB) is a key transcription factor that regulates the expression of genes involved in immune and inflammatory responses and in cell death and survival. This chapter describes in detail the method for measuring the NF-κB
Factor Assay kit试剂盒测定胞核提取物中p65蛋白与DNA靶序列结合能力。 结果表明:(1)氯化镧对TNF-α诱导的IκBa磷酸化和降解的抑制作用:TNF-α能够诱导Hela细胞中IκBa的磷酸化和降解。随着氯化镧处理的浓度不断升高,IκBa表达水平逐步升高,而磷酸化水平不断降低。表明氯化镧能够抑制TNF-α诱导的IκBa的磷酸化和表达水平的降低,且该效应随着随着氯化镧浓度的升高而逐步增强。(2)氯化镧对TNF-ct诱导的NF-κB活化的抑制作用:25―100μmol/L氯
技术资料暂无技术资料 索取技术资料









