Human Protein NDNF,NDNF ELISA

Human Protein NDNF, NDNF ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Protein NDNF,Neuron-derived neurotrophic factor, protein NDNF; C4orf31; fibronectin type-III domain-containing protein C4orf31; neuron-derived neurotrophic factor, UNQ2748/PRO6487

Search name

Human Protein NDNF ELISA KIT ,Human Neuron-derived neurotrophic factor ELISA KIT ,Human protein NDNF ELISA KIT ,Human C4orf31 ELISA KIT ,Human fibronectin type-III domain-containing protein C4orf31 ELISA KIT ,Human neuron-derived neurotrophic factor ELISA KIT ,Human UNQ2748/PRO6487 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human protein ndnf,Protein NDNF concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein NDNF, During the reaction, Protein NDNF in the sample or standard competes with a fixed amount of biotin-labeled Protein NDNF for sites on a pre-coated Monoclonal antibody specific to Protein NDNF. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein NDNF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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