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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Myosin light polypeptide 6,MYL6
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Myosin light polypeptide 6, MYL6 ELISA KIT
Product Name:Human Myosin light polypeptide 6, MYL6 ELISA KIT
Packing:96T
Catalog No.:ELI-19963h
Gene Name:MYL6
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Myosin light polypeptide 6(MYL6
Alternative Name:MYL6,Myosin light polypeptide 6(MYL6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Myosin light polypeptide 6, MYL6 ELISA KIT allows for the in vitro quantitative determination of Myosin light polypeptide 6(MYL6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Myosin light polypeptide 6, MYL6 ELISA KIT has been pre-coated with an Myosin light polypeptide 6(MYL6 antibody specific to Myosin light polypeptide 6(MYL6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Myosin light polypeptide 6(MYL6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myosin light polypeptide 6(MYL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Myosin light polypeptide 6(MYL6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
) (DRAP1), mRNA 561 NM_021019 Homo sapiens myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1, mRNA 5933 NM_005112 Homo sapiens WD repeat domain 1 (WDR1), transcript variant 2, mRNA 809 NM_005370 Homo
就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml,所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量,排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标,形式比较固定。为满足不同客户的实际需要和研究兴趣,BD于2005年初推出了CBA Flexset
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
to free 3’-OH DNA ends in a template-independent manner, is used to label DNA strand breaks. Incorporated nucleotides are detected by a secondary antibody, conjugated with peroxidase. After substrate reaction, stained cells can be detected under light
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