Alternative Name:SEMA4B,Semaphorin-4B(SEMA4B Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Semaphorin- 4B, SEMA4B ELISA KIT allows for the in vitro quantitative determination of Semaphorin-4B(SEMA4B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Semaphorin- 4B, SEMA4B ELISA KIT has been pre-coated with an Semaphorin-4B(SEMA4B antibody specific to Semaphorin-4B(SEMA4B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Semaphorin-4B(SEMA4B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Semaphorin-4B(SEMA4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Semaphorin-4B(SEMA4B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!