- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-20762h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Protein kinase C-binding protein NELL1,NELL1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Protein kinase C- binding protein NELL1, NELL1 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Human NELL1 ELISA KIT ,Human NELL-1 ELISA KIT ,Human IDH3GL ELISA KIT ,Human NRP1 ELISA KIT ,Human nel-related protein 1 ELISA KIT ,Human neural epidermal growth factor-like 1 ELISA KIT ,Human neural EGFL like 1 ELISA KIT
Intended use
Human NELL1 ELISA kit allows for the in vitro quantitative determination of Human NELL1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Human NELL1 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NELL1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NELL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NELL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human NELL1 ELISA kit Range 31.2-2000pg/ml
Human NELL1 ELISA kit Order NELL1 ELISA kit by email:
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文献和实验Analysis of Protein Kinase C Function
Protein kinase C is a key element in signal transduction, cell regulation, and tumor promotion, influencing cellular responses to many stimuli at a variety of levels, including receptors, transduction systems, ion channels, and membrane pumps
Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
PKC系统(protein kinase C system，PKC system)
( diacylglycerol, DAG)和1,4,5-三磷酸肌醇(IP3)。IP3动员细胞内钙库释放Ca2 到细胞质中与钙调蛋白结合，随后参与一系列的反应；而DAG在Ca2 的协同下激活蛋白激酶C(protein kinase C，PKC)，然后通过蛋白激酶C引起级联反应，进行细胞的应答, 故此将该系统称为PKC系统,或称为IP3、DAG、Ca2 信号通路。
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