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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Lipase maturation factor 1,LMF1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Lipase maturation factor 1, LMF1 ELISA KIT
Product Name:Human Lipase maturation factor 1, LMF1 ELISA KIT
Packing:96T
Catalog No.:ELI-20258h
Gene Name:LMF1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Lipase maturation factor 1(LMF1
Alternative Name:LMF1,Lipase maturation factor 1(LMF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Lipase maturation factor 1, LMF1 ELISA KIT allows for the in vitro quantitative determination of Lipase maturation factor 1(LMF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Lipase maturation factor 1, LMF1 ELISA KIT has been pre-coated with an Lipase maturation factor 1(LMF1 antibody specific to Lipase maturation factor 1(LMF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lipase maturation factor 1(LMF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lipase maturation factor 1(LMF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Lipase maturation factor 1(LMF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
-11, stem cell factor (SCF; also known as kit- ligand), and granulocyte-macrophage colony stimulating factor (GM-CSF) are also capable of promoting megakaryocytopoiesis and will synergize with TPO to increase megakaryocyte proliferation (reviewed in ref. 7 ).
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
In Vitro Immunogenicity Risk Assessment of Therapeutic Proteins in Preclinical Setting
-alpha (rHuEPO)and recombinant Factor VIII (rFVIII). Flow-cytometry was used to determine the maturation level of dendritic cells (DCs), a primary initiator of T-cell responses. Studies to capture T-lymphocyte proliferation upon challenge with free rFVIII
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