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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Casein kinase I isoform gamma-2,CSNK1G2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Casein kinase I isoform gamma- 2, CSNK1G2 ELISA KIT
Product Name:Human Casein kinase I isoform gamma- 2, CSNK1G2 ELISA KIT
Packing:96T
Catalog No.:ELI-19737h
Gene Name:CSNK1G2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Casein kinase I isoform gamma-2(CSNK1G2
Alternative Name:CSNK1G2,Casein kinase I isoform gamma-2(CSNK1G2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Casein kinase I isoform gamma- 2, CSNK1G2 ELISA KIT allows for the in vitro quantitative determination of Casein kinase I isoform gamma-2(CSNK1G2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Casein kinase I isoform gamma- 2, CSNK1G2 ELISA KIT has been pre-coated with an Casein kinase I isoform gamma-2(CSNK1G2 antibody specific to Casein kinase I isoform gamma-2(CSNK1G2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Casein kinase I isoform gamma-2(CSNK1G2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Casein kinase I isoform gamma-2(CSNK1G2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Casein kinase I isoform gamma-2(CSNK1G2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S. 2002b. The protein kinase complement of the human genome. Science 298:1912‐1934.
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
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