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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Suppressor of fused homolog,SUFU
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Suppressor of fused homolog, SUFU ELISA KIT
Product Name:Human Suppressor of fused homolog, SUFU ELISA KIT
Packing:96T
Catalog No.:ELI-18767h
Gene Name:SUFU
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Suppressor of fused homolog(SUFU
Alternative Name:SUFU,Suppressor of fused homolog(SUFU
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Suppressor of fused homolog, SUFU ELISA KIT allows for the in vitro quantitative determination of Suppressor of fused homolog(SUFU concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Suppressor of fused homolog, SUFU ELISA KIT has been pre-coated with an Suppressor of fused homolog(SUFU antibody specific to Suppressor of fused homolog(SUFU .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Suppressor of fused homolog(SUFU and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Suppressor of fused homolog(SUFU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Suppressor of fused homolog(SUFU in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验of VEGF protein secreted from HEK 293 cells that had been transiently transfected with the corresponding ZFPs; measured with an ELISA assay kit (Chemicon, Temecula, CA). 'Vector' indicates a control vector that encode no ZFPs. An 'irrelevant ZFP
Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
Two-hybrid analysis of genetic regulatory networks
to be involved in the disease. Recently, Reymond and Brent undertook a test of this idea (Reymond and Brent, 1995). They studied the protein encoded by the INK4 human tumor suppressor gene, p16. Wild type p16 interacts with two human Cyclin-dependent kinases, Cdk
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