- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-19500h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Methyltransferase-like protein 23,METTL23
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Methyltransferase- like protein 23, METTL23 ELISA KIT
Product Name:Human Methyltransferase- like protein 23, METTL23 ELISA KIT
Packing:96T
Catalog No.:ELI-19500h
Gene Name:METTL23
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Methyltransferase-like protein 23(METTL23
Alternative Name:METTL23,Methyltransferase-like protein 23(METTL23
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Methyltransferase- like protein 23, METTL23 ELISA KIT allows for the in vitro quantitative determination of Methyltransferase-like protein 23(METTL23 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Methyltransferase- like protein 23, METTL23 ELISA KIT has been pre-coated with an Methyltransferase-like protein 23(METTL23 antibody specific to Methyltransferase-like protein 23(METTL23 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Methyltransferase-like protein 23(METTL23 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Methyltransferase-like protein 23(METTL23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Methyltransferase-like protein 23(METTL23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验2.Quantitate Protein Concentration Quantitate proteins in 15 ml aliquots of lysates (in duplicate)by using a Bradford assay (I have been using the Biorad kit),using BSA as standard.Don't forget to add 15 ml LB to each BSA sample and to the blank tube
the solution through a 20 gauge needle x2, followed by a 25 gauge needle x2, and freeze in an ETOH/dry ice bath. Repeat freeze/thaw cycle x2 (3 times total), and store lysate at -70°C until ready to quantitate proteins. 2. Quantitate Protein Concentration
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation
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