Human Ribonuclease- like protein 12, RNASE12 ELISA KIT
Product Name:Human Ribonuclease- like protein 12, RNASE12 ELISA KIT Packing:96T
Catalog No.:ELI-18518h
Gene Name:RNASE12
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Ribonuclease-like protein 12(RNASE12
Alternative Name:RNASE12,Ribonuclease-like protein 12(RNASE12 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Ribonuclease- like protein 12, RNASE12 ELISA KIT allows for the in vitro quantitative determination of Ribonuclease-like protein 12(RNASE12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Ribonuclease- like protein 12, RNASE12 ELISA KIT has been pre-coated with an Ribonuclease-like protein 12(RNASE12 antibody specific to Ribonuclease-like protein 12(RNASE12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ribonuclease-like protein 12(RNASE12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ribonuclease-like protein 12(RNASE12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ribonuclease-like protein 12(RNASE12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!