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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Ribonuclease-like protein 10,RNASE10
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Ribonuclease- like protein 10, RNASE10 ELISA KIT
Product Name:Human Ribonuclease- like protein 10, RNASE10 ELISA KIT
Packing:96T
Catalog No.:ELI-18517h
Gene Name:RNASE10
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Ribonuclease-like protein 10(RNASE10
Alternative Name:RNASE10,Ribonuclease-like protein 10(RNASE10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ribonuclease- like protein 10, RNASE10 ELISA KIT allows for the in vitro quantitative determination of Ribonuclease-like protein 10(RNASE10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ribonuclease- like protein 10, RNASE10 ELISA KIT has been pre-coated with an Ribonuclease-like protein 10(RNASE10 antibody specific to Ribonuclease-like protein 10(RNASE10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ribonuclease-like protein 10(RNASE10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ribonuclease-like protein 10(RNASE10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ribonuclease-like protein 10(RNASE10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验in the same volume of Buffer 1 as the initial volume transferred from the vial in step 2. 2. Sample Preparation Preparation of MNC from Buffy Coat to Obtain Low Platelet Numbers 1) Dilute 10 – 18 ml buffy coat with Buffer 2 to a total
Ribonuclease inhibitor (RI) is a cytoplasmic protein of ∼50 kDa that tightly binds and inhibits ribonucleases (RNases) from the pancreatic superfamily (1 ). Diverse RNases with very limited sequence similarities, including RNase
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
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