Alternative Name:CAD,CAD protein(CAD Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human CAD protein, CAD ELISA KIT allows for the in vitro quantitative determination of CAD protein(CAD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human CAD protein, CAD ELISA KIT has been pre-coated with an CAD protein(CAD antibody specific to CAD protein(CAD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CAD protein(CAD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CAD protein(CAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of CAD protein(CAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!