- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-17528h
- 2025年08月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Werner syndrome ATP-dependent helicase,WRN
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Werner syndrome ATP- dependent helicase, WRN ELISA KIT
Product Name:Human Werner syndrome ATP- dependent helicase, WRN ELISA KIT
Packing:96T
Catalog No.:ELI-17528h
Gene Name:WRN
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Werner syndrome ATP-dependent helicase(WRN
Alternative Name:WRN,Werner syndrome ATP-dependent helicase(WRN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Werner syndrome ATP- dependent helicase, WRN ELISA KIT allows for the in vitro quantitative determination of Werner syndrome ATP-dependent helicase(WRN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Werner syndrome ATP- dependent helicase, WRN ELISA KIT has been pre-coated with an Werner syndrome ATP-dependent helicase(WRN antibody specific to Werner syndrome ATP-dependent helicase(WRN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Werner syndrome ATP-dependent helicase(WRN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Werner syndrome ATP-dependent helicase(WRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Werner syndrome ATP-dependent helicase(WRN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验, Haverkamp TH, van Luenen HG, and Plasterk RH. (1999). Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNase D. Cell 99: 133-141. (Abstract) Grishok A, Pasquinelli AE, Conte D, Li N
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Luenen, H. G. & Plasterk, R. H. mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell 99, 133-141 (1999). 57. Sijen, T. & Plasterk, R. Transposon silencing
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