- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-17761h
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Ubiquitin carboxyl-terminal hydrolase 48,USP48
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ubiquitin carboxyl- terminal hydrolase 48, USP48 ELISA KIT
Product Name:Human Ubiquitin carboxyl- terminal hydrolase 48, USP48 ELISA KIT
Packing:96T
Catalog No.:ELI-17761h
Gene Name:USP48
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Ubiquitin carboxyl-terminal hydrolase 48(USP48
Alternative Name:USP48,Ubiquitin carboxyl-terminal hydrolase 48(USP48
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin carboxyl- terminal hydrolase 48, USP48 ELISA KIT allows for the in vitro quantitative determination of Ubiquitin carboxyl-terminal hydrolase 48(USP48 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin carboxyl- terminal hydrolase 48, USP48 ELISA KIT has been pre-coated with an Ubiquitin carboxyl-terminal hydrolase 48(USP48 antibody specific to Ubiquitin carboxyl-terminal hydrolase 48(USP48 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase 48(USP48 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase 48(USP48, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin carboxyl-terminal hydrolase 48(USP48 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
6.3 ) Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype 20× ubiquitylation buffer 2 for E3 assays (see recipe ) Mutant ubiquitins: lysine‐less (K0), K48R, K63R, K48 only, K63 only (BioMol
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
, and a carboxy-terminal truncated isoform, apoB-48, respectively. In mice, editing of apoB mRNA occurs in both the liver and jejunum: apoB-48 is the predominant protein form in the jejunum and both apoB-48 and apoB-100 are expressed in the liver. Heterozygous
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