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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Toll/interleukin-1 receptor domain-containing adapter pr
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Toll/interleukin- 1 receptor domain- containing adapter protein, TIRAP ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TIRAP,BACTS1; Mal; MyD88-2; wyatt; MyD88 adapter-like protein; Toll-like receptor adaptor protein; adapter protein wyatt; adaptor protein Wyatt; toll/interleukin-1 receptor domain-containing adapter protein; toll-interleukin 1 receptor (TIR) domain containing adaptor protein
Search name
Human TIRAP ELISA KIT ,Human BACTS1 ELISA KIT ,Human Mal ELISA KIT ,Human MyD88-2 ELISA KIT ,Human wyatt ELISA KIT ,Human MyD88 adapter-like protein ELISA KIT ,Human Toll-like receptor adaptor protein ELISA KIT ,Human adapter protein wyatt ELISA KIT ,Human adaptor protein Wyatt ELISA KIT ,Human toll/interleukin-1 receptor domain-containing adapter protein ELISA KIT ,Human toll-interleukin 1 receptor domain containing adaptor protein ELISA KIT ,Human TIR domain containing adaptor protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Toll/interleukin- 1 receptor domain- containing adapter protein, TIRAP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIRAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TIRAP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TIRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TIRAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验【信息】The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling
Review Nature Reviews Immunology 7, 353-364 (May 2007) | doi:10.1038/nri2079 The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling Luke A. J. O'Neill1 & Andrew G. Bowie1 About the authors Summary TLR (Toll
Human progesterone receptor (PR) is a member of the nuclear hormone receptor superfamily of transcriptional activators, which share a common modular structure consisting of a C-terminal ligand-binding domain (LBD), a highly conserved
Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures
. In the context of Toll-interleukin-1 receptor (TIR) domain containing proteins, we describe here a series of the more common and user-friendly bioinformatic tools available as internet-based resources. These will enable the identification and alignment of protein
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