- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-17058h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Ubiquitin-conjugating enzyme E2 H,UBE2H
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ubiquitin- conjugating enzyme E2 H, UBE2H ELISA KIT
Product Name:Human Ubiquitin- conjugating enzyme E2 H, UBE2H ELISA KIT
Packing:96T
Catalog No.:ELI-17058h
Gene Name:UBE2H
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Ubiquitin-conjugating enzyme E2 H(UBE2H
Alternative Name:UBE2H,Ubiquitin-conjugating enzyme E2 H(UBE2H
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin- conjugating enzyme E2 H, UBE2H ELISA KIT allows for the in vitro quantitative determination of Ubiquitin-conjugating enzyme E2 H(UBE2H concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin- conjugating enzyme E2 H, UBE2H ELISA KIT has been pre-coated with an Ubiquitin-conjugating enzyme E2 H(UBE2H antibody specific to Ubiquitin-conjugating enzyme E2 H(UBE2H .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin-conjugating enzyme E2 H(UBE2H and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-conjugating enzyme E2 H(UBE2H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin-conjugating enzyme E2 H(UBE2H in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
in Insect Cells Using a Baculovirus System Alternate Protocol 11: Preparation of Rabbit E1 Support Protocol 4: Generation of Empty Bacterial Lysates Generation of Ubiquitin‐Conjugating Enzymes (E2s) Basic Protocol 22: Expression of E2s in E. coli
【分享】Human press METHODS IN MOLECULAR BIOLOGY
by Selinsky, Barry S. 2003 229.Lentivirus Gene Engineering Protocols. Edited by Federico, Maurizio 2003 230.Directed Enzyme Evolution Screening and Selection Methods. Edited by Arnold, Frances H. 2003 231.Directed Evolution LibraryCreation
的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述，固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定（enzyme linked immunosorbent assay），简称ELISA，在国内有译作酶联免疫吸附试验或酶标，已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
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