- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-16456h
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Serine/threonine-protein kinase Nek7,NEK7
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Serine/threonine- protein kinase Nek7, NEK7 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NEK7,serine/threonine-protein kinase Nek7; NIMA (never in mitosis gene a)-related kinase 7; never in mitosis A-related kinase 7; nimA-related protein kinase 7; NIMA-related kinase 7
Search name
Human NEK7 ELISA KIT ,Human serine/threonine-protein kinase Nek7 ELISA KIT ,Human NIMA (never in mitosis gene a)-related kinase 7 ELISA KIT ,Human never in mitosis A-related kinase 7 ELISA KIT ,Human nimA-related protein kinase 7 ELISA KIT ,Human NIMA-related kinase 7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Serine/threonine- protein kinase Nek7, NEK7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEK7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NEK7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NEK7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NEK7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
family (4 ), however, in vitro DNA-PK acts as a serine/threonine protein kinase (5 –3 ). The physiological substrates of DNA-PK are not known, however in vitro DNA-PK is known to phosphorylate many proteins including hsp90, SV40 large T antigen, serum
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
Use of Animal Models to Understand Human Muscular Dystrophy
, respectively; laminin α2 -chain mutations have been shown to result in congenital MD (4 ). Defects in non-DGC components that cause include the calpain 3 in LGMD2A (4 ), expansion of a CTG repeat in the 3′ untranslated region of a serine threonine kinase
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