- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-15533h
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
DNA repair protein RAD51 homolog 1,RAD51
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT
Product Name:Human DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT
Packing:96T
Catalog No.:ELI-15533h
Gene Name:RAD51
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:DNA repair protein RAD51 homolog 1(RAD51
Alternative Name:RAD51,DNA repair protein RAD51 homolog 1(RAD51
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT allows for the in vitro quantitative determination of DNA repair protein RAD51 homolog 1(RAD51 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT has been pre-coated with an DNA repair protein RAD51 homolog 1(RAD51 antibody specific to DNA repair protein RAD51 homolog 1(RAD51 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA repair protein RAD51 homolog 1(RAD51 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA repair protein RAD51 homolog 1(RAD51, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA repair protein RAD51 homolog 1(RAD51 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
。 Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5120 Assay for Human Rad51-Mediated DNA Displacement Loop Formation Steven Raynard and Patrick Sung1 Department of Molecular Biophysics and Biochemistry, Yale
Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Rad51-mediated pairing between homologous DNA sequences during homologous recombination (HR) plays pivotal roles in DNA double-strand break repair. The multi-step process occurs through cooperation of Rad51 and a number of accessory protein
In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
/Rad51 family of proteins are the key enzymes in this homology-directed repair pathway, as they perform DNA strand invasion and exchange, in concert with a host of ancillary factors. In vitro, the RecA/Rad51 family of proteins share similar enzymatic
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