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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Proline-rich protein 20D,PRR20D
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Proline- rich protein 20D, PRR20D ELISA KIT
Product Name:Human Proline- rich protein 20D, PRR20D ELISA KIT
Packing:96T
Catalog No.:ELI-15420h
Gene Name:PRR20D
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Proline-rich protein 20D(PRR20D
Alternative Name:PRR20D,Proline-rich protein 20D(PRR20D
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Proline- rich protein 20D, PRR20D ELISA KIT allows for the in vitro quantitative determination of Proline-rich protein 20D(PRR20D concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Proline- rich protein 20D, PRR20D ELISA KIT has been pre-coated with an Proline-rich protein 20D(PRR20D antibody specific to Proline-rich protein 20D(PRR20D .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Proline-rich protein 20D(PRR20D and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proline-rich protein 20D(PRR20D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Proline-rich protein 20D(PRR20D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Purification and Proteomic Analysis of 20S Proteasomes from Human Cells
of proteomic approaches to study the subunit composition of 20S proteasome complexes purified from human cells. An immunoaffinity purification method is presented. The separation of all 20S proteasome subunits by 2D gel electrophoresis and the subunit
玉米Proline responding 1(pro1)突变在蛋白合成和细胞周期调控中起到关键作用
技术有限公司提供)。结果分析pro1突变体造成粉质胚乳和苗期致死表型1975年,Giuseppe Gavazzi等人描述了一种条件致死型的,由单基因控制的玉米籽粒的隐性突变体。纯合的pro1突变籽粒的外观呈现出发育不良、皱缩以及不透光的opaque表型(Figure 1A,B,C,D)以及淀粉、油脂和蛋白合成的减少(Figure 1G,H,I)。若将突变籽粒进行种植,与正常植株相比较,pro1突变体幼苗的植株矮小,株高只有同一时期野生型株高的20%左右,根长只有同一时期野生型株高的25%左右
each of seven different α and β subunits arranged into four stacked rings (α7 β7 β7 α7 ). Separation by two-dimensional (2D) gel electrophoresis of the human erythrocytes 20S proteasome subunits and mass spectrometry (MS) identification of all the observed spots reveal
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