Human Proline dehydrogenase 1, mitochondrial, PRODH ELISA KIT
Product Name:Human Proline dehydrogenase 1, mitochondrial, PRODH ELISA KIT
Packing:96T
Catalog No.:ELI-15412h
Gene Name:PRODH
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Proline dehydrogenase 1(mitochondrial(PRODH
Alternative Name:PRODH,Proline dehydrogenase 1(mitochondrial(PRODH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Proline dehydrogenase 1, mitochondrial, PRODH ELISA KIT allows for the in vitro quantitative determination of Proline dehydrogenase 1(mitochondrial(PRODH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Proline dehydrogenase 1, mitochondrial, PRODH ELISA KIT has been pre-coated with an Proline dehydrogenase 1(mitochondrial(PRODH antibody specific to Proline dehydrogenase 1(mitochondrial(PRODH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Proline dehydrogenase 1(mitochondrial(PRODH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proline dehydrogenase 1(mitochondrial(PRODH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Proline dehydrogenase 1(mitochondrial(PRODH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!