- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-14934h
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Rab GTPase-activating protein 1-like,RABGAP1L
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Rab GTPase- activating protein 1- like, RABGAP1L ELISA KIT
Product Name:Human Rab GTPase- activating protein 1- like, RABGAP1L ELISA KIT
Packing:96T
Catalog No.:ELI-14934h
Gene Name:RABGAP1L
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Rab GTPase-activating protein 1-like(RABGAP1L
Alternative Name:RABGAP1L,Rab GTPase-activating protein 1-like(RABGAP1L
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rab GTPase- activating protein 1- like, RABGAP1L ELISA KIT allows for the in vitro quantitative determination of Rab GTPase-activating protein 1-like(RABGAP1L concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rab GTPase- activating protein 1- like, RABGAP1L ELISA KIT has been pre-coated with an Rab GTPase-activating protein 1-like(RABGAP1L antibody specific to Rab GTPase-activating protein 1-like(RABGAP1L .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rab GTPase-activating protein 1-like(RABGAP1L and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rab GTPase-activating protein 1-like(RABGAP1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rab GTPase-activating protein 1-like(RABGAP1L in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
of artificial transcription factors were created and used to select in vivo protein-DNA interactions that confer a desired phenotype or molecular function to human cells through the activation of one or more genomic loci. Results and discussion
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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