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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Rho GTPase-activating protein 1,ARHGAP1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Rho GTPase- activating protein 1, ARHGAP1 ELISA KIT
Product Name:Human Rho GTPase- activating protein 1, ARHGAP1 ELISA KIT
Packing:96T
Catalog No.:ELI-14573h
Gene Name:ARHGAP1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Rho GTPase-activating protein 1(ARHGAP1
Alternative Name:ARHGAP1,Rho GTPase-activating protein 1(ARHGAP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 1, ARHGAP1 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 1(ARHGAP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 1, ARHGAP1 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 1(ARHGAP1 antibody specific to Rho GTPase-activating protein 1(ARHGAP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 1(ARHGAP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 1(ARHGAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 1(ARHGAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验of artificial transcription factors were created and used to select in vivo protein-DNA interactions that confer a desired phenotype or molecular function to human cells through the activation of one or more genomic loci. Results and discussion
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
(protein disulfide isomerase; thyroid hormone binding protein p55) (P4HB), mRNA 2719 NM_004584 Homo sapiens RAD9 homolog (S. pombe) (RAD9), mRNA 860 NM_004952 Homo sapiens ephrin-A3 (EFNA3), mRNA 505 NM_004308 Homo sapiens Rho GTPase activating
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
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