Human Minor histocompatibility antigen H13, HM13 ELISA KIT
Product Name:Human Minor histocompatibility antigen H13, HM13 ELISA KIT Packing:96T
Catalog No.:ELI-14457h
Gene Name:HM13
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Minor histocompatibility antigen H13(HM13
Alternative Name:HM13,Minor histocompatibility antigen H13(HM13 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Minor histocompatibility antigen H13, HM13 ELISA KIT allows for the in vitro quantitative determination of Minor histocompatibility antigen H13(HM13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Minor histocompatibility antigen H13, HM13 ELISA KIT has been pre-coated with an Minor histocompatibility antigen H13(HM13 antibody specific to Minor histocompatibility antigen H13(HM13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Minor histocompatibility antigen H13(HM13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Minor histocompatibility antigen H13(HM13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Minor histocompatibility antigen H13(HM13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!