Human RGM domain family member

Human RGM domain family member B, RGMB ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

RGMB,DRAGON,DRG11-responsive axonal guidance and outgrowth of neurite; RGM domain family member B; repulsive guidance molecule B; RGM domain family, member B

Search name

Human RGMB ELISA KIT ,Human DRAGON ELISA KIT ,Human DRG11-responsive axonal guidance and outgrowth of neurite ELISA KIT ,Human RGM domain family member B ELISA KIT ,Human repulsive guidance molecule B ELISA KIT ,Human RGM domain family member B ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human RGM domain family member B, RGMB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to RGMB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RGMB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RGMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RGMB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human RGMB ELISA kit  Range 0.15-10ng/ml

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