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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
2-acylglycerol O-acyltransferase 3,MOGAT3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human 2- acylglycerol O- acyltransferase 3, MOGAT3 ELISA KIT
Product Name:Human 2- acylglycerol O- acyltransferase 3, MOGAT3 ELISA KIT
Packing:96T
Catalog No.:ELI-14125h
Gene Name:MOGAT3
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:2-acylglycerol O-acyltransferase 3(MOGAT3
Alternative Name:MOGAT3,2-acylglycerol O-acyltransferase 3(MOGAT3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human 2- acylglycerol O- acyltransferase 3, MOGAT3 ELISA KIT allows for the in vitro quantitative determination of 2-acylglycerol O-acyltransferase 3(MOGAT3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human 2- acylglycerol O- acyltransferase 3, MOGAT3 ELISA KIT has been pre-coated with an 2-acylglycerol O-acyltransferase 3(MOGAT3 antibody specific to 2-acylglycerol O-acyltransferase 3(MOGAT3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 2-acylglycerol O-acyltransferase 3(MOGAT3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 2-acylglycerol O-acyltransferase 3(MOGAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 2-acylglycerol O-acyltransferase 3(MOGAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Methods for Studying Rodent Intestinal Lipoprotein Production and Metabolism
cannula (highly recommended): o.d 0.80 × i.d. 0.5 mm (Critchley Electrical Products Pty) Duodenal infusion tube (e.g., 0.040‐in. i.d. × 0.085‐in. o.d; VWR, cat. no. 60985‐708
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