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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Methyltransferase-like protein 16,METTL16
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Methyltransferase- like protein 16, METTL16 ELISA KIT
Product Name:Human Methyltransferase- like protein 16, METTL16 ELISA KIT
Packing:96T
Catalog No.:ELI-13018h
Gene Name:METTL16
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Methyltransferase-like protein 16(METTL16
Alternative Name:METTL16,Methyltransferase-like protein 16(METTL16
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Methyltransferase- like protein 16, METTL16 ELISA KIT allows for the in vitro quantitative determination of Methyltransferase-like protein 16(METTL16 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Methyltransferase- like protein 16, METTL16 ELISA KIT has been pre-coated with an Methyltransferase-like protein 16(METTL16 antibody specific to Methyltransferase-like protein 16(METTL16 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Methyltransferase-like protein 16(METTL16 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Methyltransferase-like protein 16(METTL16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Methyltransferase-like protein 16(METTL16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
interactions. Nat. Biotechnol. 18:847‐851. Ramani, A.K., Bunescu, R.C., Mooney, R.J., and Marcotte, E.M. 2005. Consolidating the set of known human protein‐protein interactions
Functional Assays on High‐Content Protein Microarrays
Substrate Profiling Assay on the ProtoArray Human Protein Array Basic Protocol 5: Methyltransferase Profiling Assay on the ProtoArray Human Protein Array Reagents and Solutions Commentary
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