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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Heat shock protein beta-11,HSPB11
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Heat shock protein beta- 11, HSPB11 ELISA KIT
Product Name:Human Heat shock protein beta- 11, HSPB11 ELISA KIT
Packing:96T
Catalog No.:ELI-12975h
Gene Name:HSPB11
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Heat shock protein beta-11(HSPB11
Alternative Name:HSPB11,Heat shock protein beta-11(HSPB11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Heat shock protein beta- 11, HSPB11 ELISA KIT allows for the in vitro quantitative determination of Heat shock protein beta-11(HSPB11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Heat shock protein beta- 11, HSPB11 ELISA KIT has been pre-coated with an Heat shock protein beta-11(HSPB11 antibody specific to Heat shock protein beta-11(HSPB11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Heat shock protein beta-11(HSPB11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heat shock protein beta-11(HSPB11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Heat shock protein beta-11(HSPB11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
Cellular stress proteins like the classical heat-shock proteins (HSPs) hsp70 (1 –3 ), hsp90 (3 ) and hsp110 (4 ); the glucose-regulated proteins gp96/GRP94 (3 ,5 ) and grp170 (4 ); as well as the endoplasmic chaperone calreticulin
Heat shock proteins: biological functions and clinical application as personalized vaccines for human cancer. Cancer Immunol Immunother. 2004 Mar;53(3):227-33.PMID: 14689240 Heat shock proteins (HSPs) are a large family of proteins
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