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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Long- chain- fatty- acid- - CoA ligase 5, ACSL5
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Long- chain- fatty- acid- - CoA ligase 5, ACSL5 ELISA KIT
Product Name:Human Long- chain- fatty- acid- - CoA ligase 5, ACSL5 ELISA KIT
Packing:96T
Catalog No.:ELI-12073h
Gene Name:ACSL5
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Long-chain-fatty-acid--CoA ligase 5(ACSL5
Alternative Name:ACSL5,Long-chain-fatty-acid--CoA ligase 5(ACSL5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Long- chain- fatty- acid- - CoA ligase 5, ACSL5 ELISA KIT allows for the in vitro quantitative determination of Long-chain-fatty-acid--CoA ligase 5(ACSL5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Long- chain- fatty- acid- - CoA ligase 5, ACSL5 ELISA KIT has been pre-coated with an Long-chain-fatty-acid--CoA ligase 5(ACSL5 antibody specific to Long-chain-fatty-acid--CoA ligase 5(ACSL5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Long-chain-fatty-acid--CoA ligase 5(ACSL5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Long-chain-fatty-acid--CoA ligase 5(ACSL5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Long-chain-fatty-acid--CoA ligase 5(ACSL5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验microsomal long‐chain fatty acid and nafenopin‐CoA ligases. Chemico‐Biol. Interact. 90:215‐223. Rodriguez‐Aparicio, L.B., Reglero, A., Martinex‐Blanco, H., and Luengo, J.M. 1991
Mammalian Fatty Acid Elongases
Very long chain fatty acids confer functional diversity on cells by variations in their chain length and degree of unsaturation. Microsomal fatty acid elongation represents the major pathway for determining the chain length of saturated
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