Product Name:Human AF4/FMR2 family member 2, AFF2 ELISA KIT Packing:96T
Catalog No.:ELI-11956h
Gene Name:AFF2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:AF4(FMR2 family member 2(AFF2
Alternative Name:AFF2,AF4(FMR2 family member 2(AFF2 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human AF4/FMR2 family member 2, AFF2 ELISA KIT allows for the in vitro quantitative determination of AF4(FMR2 family member 2(AFF2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human AF4/FMR2 family member 2, AFF2 ELISA KIT has been pre-coated with an AF4(FMR2 family member 2(AFF2 antibody specific to AF4(FMR2 family member 2(AFF2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AF4(FMR2 family member 2(AFF2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AF4(FMR2 family member 2(AFF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of AF4(FMR2 family member 2(AFF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!