- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-11945h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
RAC- beta serine/threonine- protein kinase, AKT2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human RAC- beta serine/threonine- protein kinase, AKT2 ELISA KIT
Product Name:Human RAC- beta serine/threonine- protein kinase, AKT2 ELISA KIT
Packing:96T
Catalog No.:ELI-11945h
Gene Name:AKT2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:RAC-beta serine(threonine-protein kinase(AKT2
Alternative Name:AKT2,RAC-beta serine(threonine-protein kinase(AKT2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human RAC- beta serine/threonine- protein kinase, AKT2 ELISA KIT allows for the in vitro quantitative determination of RAC-beta serine(threonine-protein kinase(AKT2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human RAC- beta serine/threonine- protein kinase, AKT2 ELISA KIT has been pre-coated with an RAC-beta serine(threonine-protein kinase(AKT2 antibody specific to RAC-beta serine(threonine-protein kinase(AKT2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for RAC-beta serine(threonine-protein kinase(AKT2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAC-beta serine(threonine-protein kinase(AKT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of RAC-beta serine(threonine-protein kinase(AKT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
. 1. Introduction Glycogen synthase kinase (GSK)-3 is a serine/threonine proteinkinase which was first described as a component of insulin-mediatedsignaling pathway (1) . There are two homologous mammalianisoforms, encoded by different genes, GSK-3a and GSK
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