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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Armadillo repeat- containing protein 10, ARMC10
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Armadillo repeat- containing protein 10, ARMC10 ELISA KIT
Product Name:Human Armadillo repeat- containing protein 10, ARMC10 ELISA KIT
Packing:96T
Catalog No.:ELI-11848h
Gene Name:ARMC10
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Armadillo repeat-containing protein 10(ARMC10
Alternative Name:ARMC10,Armadillo repeat-containing protein 10(ARMC10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Armadillo repeat- containing protein 10, ARMC10 ELISA KIT allows for the in vitro quantitative determination of Armadillo repeat-containing protein 10(ARMC10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Armadillo repeat- containing protein 10, ARMC10 ELISA KIT has been pre-coated with an Armadillo repeat-containing protein 10(ARMC10 antibody specific to Armadillo repeat-containing protein 10(ARMC10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Armadillo repeat-containing protein 10(ARMC10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Armadillo repeat-containing protein 10(ARMC10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Armadillo repeat-containing protein 10(ARMC10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Elite ABC kit) in 1 mL of PBS containing 0.1% BSA for 10 min at room temperature. ix. After incubation with secondary antibodies, rinse slides twice in PBS containing 0.1% BSA (10 min each). Remove excess liquid
Fastfilter Plasmid Maxi Kit Spin Protocol
the flow-through and reuse the collection tube in Step 11. 11. Add 10 ml Buffer HB to the Maxi column and centrifuge as above. This step ensures that residual protein contamination is removed and must be included for downstream applications requiring
Fastfilter Plasmid Midi Kit Spin Protocol
, DNA Wash Buffer must be brought to room temperature before use. 13. Optional Step: Repeat wash step with another 3.5 ml of DNA Wash Buffer. Centrifuge as above and discard fluid. 14. Centrifuge the empty capped column for 10-15 min
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