- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-12834h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Potassium channel subfamily K member 15, KCNK15
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Potassium channel subfamily K member 15, KCNK15 ELISA KIT
Product Name:Human Potassium channel subfamily K member 15, KCNK15 ELISA KIT
Packing:96T
Catalog No.:ELI-12834h
Gene Name:KCNK15
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Potassium channel subfamily K member 15(KCNK15
Alternative Name:KCNK15,Potassium channel subfamily K member 15(KCNK15
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Potassium channel subfamily K member 15, KCNK15 ELISA KIT allows for the in vitro quantitative determination of Potassium channel subfamily K member 15(KCNK15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Potassium channel subfamily K member 15, KCNK15 ELISA KIT has been pre-coated with an Potassium channel subfamily K member 15(KCNK15 antibody specific to Potassium channel subfamily K member 15(KCNK15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Potassium channel subfamily K member 15(KCNK15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Potassium channel subfamily K member 15(KCNK15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Potassium channel subfamily K member 15(KCNK15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Characterization of Potassium Channel Binding
Figure 1.17.1 Displacement of [3 H]P 1075 whole‐cell binding to KATP channels in rat A10 cells using P 1075 and two potassium channel–opener compounds. The data was fitted using GraphPad Prism program. Figure kindly contributed by R. Davis Taber
Introduction of human 14-3-3 proteins
-1,was markedly increased following T cell activation.The use of truncated 14-3-3tau fusion proteins demonstrated that the15℃terminal residues are required for the association between 14-3-3 and three of its target proteins,namely,Cbl,Raf-1,and PI3-K.The findings
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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