- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-12499h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Keratin, type I cuticular Ha8, KRT38
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Keratin, type I cuticular Ha8, KRT38 ELISA KIT
Product Name:Human Keratin, type I cuticular Ha8, KRT38 ELISA KIT
Packing:96T
Catalog No.:ELI-12499h
Gene Name:KRT38
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Keratin(type I cuticular Ha8(KRT38
Alternative Name:KRT38,Keratin(type I cuticular Ha8(KRT38
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Keratin, type I cuticular Ha8, KRT38 ELISA KIT allows for the in vitro quantitative determination of Keratin(type I cuticular Ha8(KRT38 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Keratin, type I cuticular Ha8, KRT38 ELISA KIT has been pre-coated with an Keratin(type I cuticular Ha8(KRT38 antibody specific to Keratin(type I cuticular Ha8(KRT38 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Keratin(type I cuticular Ha8(KRT38 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Keratin(type I cuticular Ha8(KRT38, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Keratin(type I cuticular Ha8(KRT38 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
Studies of the Ubiquitin Proteasome System
or protocol 4023 ) or purified ( protocol 38 or protocol 3913 ) or commercially available 10× thiolester buffer (see recipe ) [32 P]ubiquitin ( protocol 26
： PI和Hoechst33342都是sigma公司的粉剂，不是kit，2、具体方法： 将PI和Hoechst33342的终浓度调到10μg/mg就行，用PBS，Hank's液、生理盐水或D液任何一种溶解都可以。Hoechst33342染色37℃10min就达到饱和，PI在4℃10~20min就行。3、文献出处： 《细胞实验指南》上册，p102－125，现代生物技术译从－－科学出版社， 有好几篇文献，只列出其中的一个： Ha HC, Snyder SH. Poly(ADP-ribose
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