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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Alanyl- tRNA editing protein Aarsd1, AARSD1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Alanyl- tRNA editing protein Aarsd1, AARSD1 ELISA KIT
Product Name:Human Alanyl- tRNA editing protein Aarsd1, AARSD1 ELISA KIT
Packing:96T
Catalog No.:ELI-12235h
Gene Name:AARSD1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Alanyl-tRNA editing protein Aarsd1(AARSD1
Alternative Name:AARSD1,Alanyl-tRNA editing protein Aarsd1(AARSD1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Alanyl- tRNA editing protein Aarsd1, AARSD1 ELISA KIT allows for the in vitro quantitative determination of Alanyl-tRNA editing protein Aarsd1(AARSD1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Alanyl- tRNA editing protein Aarsd1, AARSD1 ELISA KIT has been pre-coated with an Alanyl-tRNA editing protein Aarsd1(AARSD1 antibody specific to Alanyl-tRNA editing protein Aarsd1(AARSD1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Alanyl-tRNA editing protein Aarsd1(AARSD1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alanyl-tRNA editing protein Aarsd1(AARSD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Alanyl-tRNA editing protein Aarsd1(AARSD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
Overview of Formation of G‐Quadruplex Structures
. Phan, A.T., Kuryavyi, V., Burge, S., Neidle, S., and Patel, D.J. 2007b. Structure of an unprecedented G‐quadruplex scaffold in the human c‐kit promoter. J. Am. Chem. Soc. 129: 4386‐4392
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