- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-11306h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Serine/threonine- protein kinase ATR, ATR
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Serine/threonine- protein kinase ATR, ATR ELISA KIT
Product Name:Human Serine/threonine- protein kinase ATR, ATR ELISA KIT
Packing:96T
Catalog No.:ELI-11306h
Gene Name:ATR
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Serine(threonine-protein kinase ATR(ATR
Alternative Name:ATR,Serine(threonine-protein kinase ATR(ATR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Serine/threonine- protein kinase ATR, ATR ELISA KIT allows for the in vitro quantitative determination of Serine(threonine-protein kinase ATR(ATR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Serine/threonine- protein kinase ATR, ATR ELISA KIT has been pre-coated with an Serine(threonine-protein kinase ATR(ATR antibody specific to Serine(threonine-protein kinase ATR(ATR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Serine(threonine-protein kinase ATR(ATR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serine(threonine-protein kinase ATR(ATR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Serine(threonine-protein kinase ATR(ATR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Identifying Inhibitors of ATM and ATR Kinase Activities
family of serine/threonine-directed kinases that contain C-terminal kinase domains bearing significant sequence homology to those of mammalian and yeast phosphoinositide 3-kinases (1 ). In response to genotoxic insults, these kinases phosphorylate
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
family (4 ), however, in vitro DNA-PK acts as a serine/threonine protein kinase (5 –3 ). The physiological substrates of DNA-PK are not known, however in vitro DNA-PK is known to phosphorylate many proteins including hsp90, SV40 large T antigen, serum
domain to that of phosphoinositide-3-kinase, these extremely large (>250 kDa) polypeptides function as serine/threonine protein kinases. The catalytic activities of these PIKK family members can now be measured in immune-complex kinase assays. This assay
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