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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Breast carcinoma- amplified sequence 1, BCAS1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Breast carcinoma- amplified sequence 1, BCAS1 ELISA KIT
Product Name:Human Breast carcinoma- amplified sequence 1, BCAS1 ELISA KIT
Packing:96T
Catalog No.:ELI-10935h
Gene Name:BCAS1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Breast carcinoma-amplified sequence 1(BCAS1
Alternative Name:BCAS1,Breast carcinoma-amplified sequence 1(BCAS1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Breast carcinoma- amplified sequence 1, BCAS1 ELISA KIT allows for the in vitro quantitative determination of Breast carcinoma-amplified sequence 1(BCAS1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Breast carcinoma- amplified sequence 1, BCAS1 ELISA KIT has been pre-coated with an Breast carcinoma-amplified sequence 1(BCAS1 antibody specific to Breast carcinoma-amplified sequence 1(BCAS1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Breast carcinoma-amplified sequence 1(BCAS1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Breast carcinoma-amplified sequence 1(BCAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Breast carcinoma-amplified sequence 1(BCAS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
5 ) or 1.5% agarose gel (GAPDH ) and quantified using ImageQuant 1.2. The 1-kbp CDH5 -specific PCR product was sequenced and shown to correspond to the expected CDH5 sequence. Luciferase assays. The human CDH5 promoter fragment (-2486 to +24) was amplified
potent transcriptional activators or repressors, respectively. These results show that the human genome encodes zinc fingers with diverse DNA-binding specificities and that these domains can be used to design sequence-specific DNA-binding proteins
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