- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-10862h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Voltage- dependent N- type calcium channel subunit alpha-
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Voltage-dependent N-type calcium channel subunit alpha-1B, CACNA1B ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Human CACNA1B ELISA KIT ,Human BIII ELISA KIT ,Human CACNL1A5 ELISA KIT ,Human CACNN ELISA KIT ,Human Cav2.2 ELISA KIT ,Human DYT23 ELISA KIT ,Human Cav2.2 voltage-gated Ca2+ channel ELISA KIT ,Human brain calcium channel III ELISA KIT ,Human calcium channel alpha12.2 subunit ELISA KIT ,Human calcium channel L type alpha-1 polypeptide ELISA KIT ,Human calcium channel voltage-dependent L type alpha 1B subunit ELISA KIT ,Human calcium channel voltage-dependent N type alpha 1B subunit ELISA KIT ,Human voltage-gated calcium channel alpha subunit Cav2.2 ELISA KIT ,Human voltage-gated calcium channel subunit alpha Cav2.2 ELISA KIT ,Human calcium voltage-gated channel subunit alpha1 B ELISA KIT
Intended use
Human CACNA1B ELISA kit allows for the in vitro quantitative determination of Human Voltage-dependent N-type calcium channel subunit alpha-1B concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Human CACNA1B ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CACNA1B, During the reaction, CACNA1B in the sample or standard competes with a fixed amount of biotin-labeled CACNA1B for sites on a pre-coated Monoclonal antibody specific to CACNA1B. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CACNA1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
Order Human CACNA1B ELISA kit by email:
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文献和实验Characterization of Calcium Channel Binding
., Patthi, S., Simerson, S., Maroufi, A., McCue, A.F., Velicelebi, G., Ellis, S.B., and Harpold, M.M. 1992. Structure and functional expression of an ω–conotoxin‐sensitive human N‐type calcium channel. Science 257:389‐395
Purification and Structure of L-Type Calcium Channels
Calcium channels are an essential part of the cellular signal trans duction system, since they produce changes in cytosolic calcium. Three types of voltage-dependent calcium channels (T, L, and N channels) have been identified
Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells
, exhibit many of the key properties found in native channels including the direct permeability of calcium, voltage-dependent Mg2+ blockade of the ion channel, and binding sites for modulators, such as Zn2+ , glycine, and polyamines (2 ). The NR1 subunit
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