Human Caspase recruitment doma

Human Caspase recruitment domain- containing protein 10, CARD10 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CARD10,BIMP1; CARMA3; Bcl10 binding protein and activator of NFKB; CARD-containing MAGUK 3 protein; CARD-containing MAGUK protein 3; carma 3; caspase recruitment domain-containing protein 10; caspase recruitment domain family member 10

Search name

Human CARD10 ELISA KIT ,Human BIMP1 ELISA KIT ,Human CARMA3 ELISA KIT ,Human Bcl10 binding protein and activator of NFKB ELISA KIT ,Human CARD-containing MAGUK 3 protein ELISA KIT ,Human CARD-containing MAGUK protein 3 ELISA KIT ,Human carma 3 ELISA KIT ,Human caspase recruitment domain-containing protein 10 ELISA KIT ,Human caspase recruitment domain family member 10 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Caspase recruitment domain- containing protein 10, CARD10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CARD10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CARD10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CARD10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CARD10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.