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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Centrosomal protein of 70 kDa, CEP70
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Centrosomal protein of 70 kDa, CEP70 ELISA KIT
Product Name:Human Centrosomal protein of 70 kDa, CEP70 ELISA KIT
Packing:96T
Catalog No.:ELI-10166h
Gene Name:CEP70
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Centrosomal protein of 70 kDa(CEP70
Alternative Name:CEP70,Centrosomal protein of 70 kDa(CEP70
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Centrosomal protein of 70 kDa, CEP70 ELISA KIT allows for the in vitro quantitative determination of Centrosomal protein of 70 kDa(CEP70 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Centrosomal protein of 70 kDa, CEP70 ELISA KIT has been pre-coated with an Centrosomal protein of 70 kDa(CEP70 antibody specific to Centrosomal protein of 70 kDa(CEP70 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Centrosomal protein of 70 kDa(CEP70 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Centrosomal protein of 70 kDa(CEP70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Centrosomal protein of 70 kDa(CEP70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Da are given in Table 1 . Table 1 R min for proteins of different mass Protein M (kDa
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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