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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
ETS homologous factor, EHF
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human ETS homologous factor, EHF ELISA KIT
Product Name:Human ETS homologous factor, EHF ELISA KIT
Packing:96T
Catalog No.:ELI-09993h
Gene Name:EHF
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:ETS homologous factor(EHF
Alternative Name:EHF,ETS homologous factor(EHF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ETS homologous factor, EHF ELISA KIT allows for the in vitro quantitative determination of ETS homologous factor(EHF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ETS homologous factor, EHF ELISA KIT has been pre-coated with an ETS homologous factor(EHF antibody specific to ETS homologous factor(EHF .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ETS homologous factor(EHF and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ETS homologous factor(EHF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of ETS homologous factor(EHF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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