- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-09549h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Zinc finger protein Gfi- 1, GFI1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Zinc finger protein Gfi- 1, GFI1 ELISA KIT
Product Name:Human Zinc finger protein Gfi- 1, GFI1 ELISA KIT
Packing:96T
Catalog No.:ELI-09549h
Gene Name:GFI1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Zinc finger protein Gfi-1(GFI1
Alternative Name:GFI1,Zinc finger protein Gfi-1(GFI1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Zinc finger protein Gfi- 1, GFI1 ELISA KIT allows for the in vitro quantitative determination of Zinc finger protein Gfi-1(GFI1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Zinc finger protein Gfi- 1, GFI1 ELISA KIT has been pre-coated with an Zinc finger protein Gfi-1(GFI1 antibody specific to Zinc finger protein Gfi-1(GFI1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Zinc finger protein Gfi-1(GFI1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Zinc finger protein Gfi-1(GFI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Zinc finger protein Gfi-1(GFI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验. For example, novel DNA-binding proteins have been created by using phage display to alter the DNA-binding specificity of a zinc-finger protein (ZFP)2-7 . Although phage display is an effective method, it is labor-intensive and time-consuming
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
Studies of the Ubiquitin Proteasome System
of E3 Enzymes Expressed in In Vitro Translation Systems Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype Basic Protocol 7: Chelation of Zinc from RING‐ and PHD‐Finger E3s Alternate Protocol 3: Inhibition of HECT Domain E
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