Human DNA (cytosine- 5)- methyltransferase 3B, DNMT3B ELISA KIT
Product Name:Human DNA (cytosine- 5)- methyltransferase 3B, DNMT3B ELISA KIT
Packing:96T
Catalog No.:ELI-09443h
Gene Name:DNMT3B
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:DNA (cytosine-5)-methyltransferase 3B(DNMT3B
Alternative Name:DNMT3B,DNA (cytosine-5)-methyltransferase 3B(DNMT3B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DNA (cytosine- 5)- methyltransferase 3B, DNMT3B ELISA KIT allows for the in vitro quantitative determination of DNA (cytosine-5)-methyltransferase 3B(DNMT3B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DNA (cytosine- 5)- methyltransferase 3B, DNMT3B ELISA KIT has been pre-coated with an DNA (cytosine-5)-methyltransferase 3B(DNMT3B antibody specific to DNA (cytosine-5)-methyltransferase 3B(DNMT3B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA (cytosine-5)-methyltransferase 3B(DNMT3B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA (cytosine-5)-methyltransferase 3B(DNMT3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA (cytosine-5)-methyltransferase 3B(DNMT3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!