- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-09167h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
ETS- related transcription factor Elf- 5, ELF5
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human ETS- related transcription factor Elf- 5, ELF5 ELISA KIT
Product Name:Human ETS- related transcription factor Elf- 5, ELF5 ELISA KIT
Packing:96T
Catalog No.:ELI-09167h
Gene Name:ELF5
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:ETS-related transcription factor Elf-5(ELF5
Alternative Name:ELF5,ETS-related transcription factor Elf-5(ELF5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ETS- related transcription factor Elf- 5, ELF5 ELISA KIT allows for the in vitro quantitative determination of ETS-related transcription factor Elf-5(ELF5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ETS- related transcription factor Elf- 5, ELF5 ELISA KIT has been pre-coated with an ETS-related transcription factor Elf-5(ELF5 antibody specific to ETS-related transcription factor Elf-5(ELF5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ETS-related transcription factor Elf-5(ELF5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ETS-related transcription factor Elf-5(ELF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of ETS-related transcription factor Elf-5(ELF5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验was unresponsive to the transcription factors (Fig. 4D). The sequence 5'-GGAA-3' (ETS-binding site-2, EBS2) is conserved between mouse and the human promoters, and in the mouse it interacts with the ETS-1 protein, a transcription factor of the ETS family expressed
interferon response elements in the promoter region, which are required for binding of the interferon-stimulated gene factor 3 (ISGF3) complex and for janus kinase/signal transducer of transcription (JAK/STAT)-pathway-mediated signaling. Expression of PRGs
were then cloned into a yeast expression plasmid that contained the Gal4-Zif268 hybrid transcription factor. In this library, zinc finger 3 (the C-terminal finger) of Zif268 was replaced with zinc-finger sequences amplified from the human genome (Fig. 1A).
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