- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产(香港）
- ELI-09104h
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Collagen alpha- 3(V) chain, COL5A3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Collagen alpha- 3(V) chain, COL5A3 ELISA KIT
Product Name:Human Collagen alpha- 3(V) chain, COL5A3 ELISA KIT
Packing:96T
Catalog No.:ELI-09104h
Gene Name:COL5A3
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Collagen alpha-3(V) chain(COL5A3
Alternative Name:COL5A3,Collagen alpha-3(V) chain(COL5A3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Collagen alpha- 3(V) chain, COL5A3 ELISA KIT allows for the in vitro quantitative determination of Collagen alpha-3(V) chain(COL5A3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Collagen alpha- 3(V) chain, COL5A3 ELISA KIT has been pre-coated with an Collagen alpha-3(V) chain(COL5A3 antibody specific to Collagen alpha-3(V) chain(COL5A3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Collagen alpha-3(V) chain(COL5A3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagen alpha-3(V) chain(COL5A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Collagen alpha-3(V) chain(COL5A3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验V294.PART II，Chapter 5 细胞粘附实验（英文版）
variations to thebasic protocols and areas where special attentions are required for successfulapplication of the methods. 2. Materials 2.1. Static Adhesion Assays 1. 96-Well enzyme-linked immunosorbent assay (ELISA) plates (Corning
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
) Materials Osteocalcin sandwich ELISA kit (Biomedical Technologies, cat. no. BT‐470) including:
Antigen Design Sera Purification Tech Sheet
-terminal alpha amine and possibly through the amine in the side-chain of Lysine, if present in the sequence. The m-Maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) is a heterobifunctional reagent that can be used to link peptides to carrier
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