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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Dual specificity protein phosphatase 14, DUSP14
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Dual specificity protein phosphatase 14, DUSP14 ELISA KIT
Product Name:Human Dual specificity protein phosphatase 14, DUSP14 ELISA KIT
Packing:96T
Catalog No.:ELI-08665h
Gene Name:DUSP14
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Dual specificity protein phosphatase 14(DUSP14
Alternative Name:DUSP14,Dual specificity protein phosphatase 14(DUSP14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Dual specificity protein phosphatase 14, DUSP14 ELISA KIT allows for the in vitro quantitative determination of Dual specificity protein phosphatase 14(DUSP14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Dual specificity protein phosphatase 14, DUSP14 ELISA KIT has been pre-coated with an Dual specificity protein phosphatase 14(DUSP14 antibody specific to Dual specificity protein phosphatase 14(DUSP14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Dual specificity protein phosphatase 14(DUSP14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dual specificity protein phosphatase 14(DUSP14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Dual specificity protein phosphatase 14(DUSP14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
, L., Kim, S., Carpino, N., Daniel, J.L., Kunapuli, S.P., Tsygankov, A.Y., and Pei, D.H. 2010. Determination of the substrate specificity of protein‐tyrosine phosphatase TULA‐2 and identification of Syk as a TULA‐2 substrate. J. Biol. Chem. 285:31268
system, or interaction trap, is one of the most versatile methods available with which to identify and establish protein?protein interactions. The dual bait system is one adaptation of the classic approach. This system facilitates the simultaneous comparison
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